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primary antibodies rabbit anti cx40  (Millipore)


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    Structured Review

    Millipore primary antibodies rabbit anti cx40
    Primary Antibodies Rabbit Anti Cx40, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+rabbit+anti+cx40/anti+cx40/pmc03188900-97-25-26
    Average 90 stars, based on 1 article reviews
    primary antibodies rabbit anti cx40 - by Bioz Stars, 2026-09
    90/100 stars

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    Generation of nonconditional Cx40A96S mice. (A) Homologous recombination of the nonconditional Cx40A96S vector with the genomic locus of the <t>Cx40</t> gene leads to the recombined allele mCx40A96SNeo. Besides the 5′ and 3′ homologous regions, the vector consists of the mutated Cx40A96S sequence followed by an IRES-eGFP construct and a neomycin resistance gene flanked by frt sites. Flp-mediated deletion of the neomycin resistance gene results in the genotype mCx40A96S. (B) Southern blot analysis with an internal probe on SacI digested liver DNA from different genotypes verified the homologous recombination in Cx40A96S mice. Detection of a 5.5-kb fragment indicates the Cx40 wild-type allele, a 7-kb fragment the Cx40A96S allele, and a 8.9-kb fragment the Cx40A96SNeo allele. (C) PCR genotyping of Cx40A96S mice results in a 290-bp fragment for the Cx40 wild-type allele, a 350-bp fragment for the Cx40A96S allele, and a 680-bp fragment for the Cx40A96SNeo allele.
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    Millipore primary antibodies rabbit anti cx40
    Generation of nonconditional Cx40A96S mice. (A) Homologous recombination of the nonconditional Cx40A96S vector with the genomic locus of the <t>Cx40</t> gene leads to the recombined allele mCx40A96SNeo. Besides the 5′ and 3′ homologous regions, the vector consists of the mutated Cx40A96S sequence followed by an IRES-eGFP construct and a neomycin resistance gene flanked by frt sites. Flp-mediated deletion of the neomycin resistance gene results in the genotype mCx40A96S. (B) Southern blot analysis with an internal probe on SacI digested liver DNA from different genotypes verified the homologous recombination in Cx40A96S mice. Detection of a 5.5-kb fragment indicates the Cx40 wild-type allele, a 7-kb fragment the Cx40A96S allele, and a 8.9-kb fragment the Cx40A96SNeo allele. (C) PCR genotyping of Cx40A96S mice results in a 290-bp fragment for the Cx40 wild-type allele, a 350-bp fragment for the Cx40A96S allele, and a 680-bp fragment for the Cx40A96SNeo allele.
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    https://www.bioz.com/product/primary+antibodies+rabbit+anti+cx40/anti+cx40/pmc03188900-97-25-26
    Average 90 stars, based on 1 article reviews
    primary antibodies rabbit anti cx40 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

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    Generation of nonconditional Cx40A96S mice. (A) Homologous recombination of the nonconditional Cx40A96S vector with the genomic locus of the Cx40 gene leads to the recombined allele mCx40A96SNeo. Besides the 5′ and 3′ homologous regions, the vector consists of the mutated Cx40A96S sequence followed by an IRES-eGFP construct and a neomycin resistance gene flanked by frt sites. Flp-mediated deletion of the neomycin resistance gene results in the genotype mCx40A96S. (B) Southern blot analysis with an internal probe on SacI digested liver DNA from different genotypes verified the homologous recombination in Cx40A96S mice. Detection of a 5.5-kb fragment indicates the Cx40 wild-type allele, a 7-kb fragment the Cx40A96S allele, and a 8.9-kb fragment the Cx40A96SNeo allele. (C) PCR genotyping of Cx40A96S mice results in a 290-bp fragment for the Cx40 wild-type allele, a 350-bp fragment for the Cx40A96S allele, and a 680-bp fragment for the Cx40A96SNeo allele.

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: The Connexin40 A96S Mutation Causes Renin-Dependent Hypertension

    doi: 10.1681/ASN.2010101047

    Figure Lengend Snippet: Generation of nonconditional Cx40A96S mice. (A) Homologous recombination of the nonconditional Cx40A96S vector with the genomic locus of the Cx40 gene leads to the recombined allele mCx40A96SNeo. Besides the 5′ and 3′ homologous regions, the vector consists of the mutated Cx40A96S sequence followed by an IRES-eGFP construct and a neomycin resistance gene flanked by frt sites. Flp-mediated deletion of the neomycin resistance gene results in the genotype mCx40A96S. (B) Southern blot analysis with an internal probe on SacI digested liver DNA from different genotypes verified the homologous recombination in Cx40A96S mice. Detection of a 5.5-kb fragment indicates the Cx40 wild-type allele, a 7-kb fragment the Cx40A96S allele, and a 8.9-kb fragment the Cx40A96SNeo allele. (C) PCR genotyping of Cx40A96S mice results in a 290-bp fragment for the Cx40 wild-type allele, a 350-bp fragment for the Cx40A96S allele, and a 680-bp fragment for the Cx40A96SNeo allele.

    Article Snippet: Primary rabbit Cx40 antibodies (BioTrend, Cologne, Germany) were diluted 1:400 in blocking solution and the cells were incubated for 1 hour at room temperature.

    Techniques: Homologous Recombination, Plasmid Preparation, Sequencing, Construct, Southern Blot

    Cx40A96S protein is expressed in the plasma membrane of renin-expressing cells. Immunoreactivity of Cx40 (red), renin (green), and α-smooth muscle actin (blue) in sections of homozygous Cx40A96S kidneys. G indicates glomerulus; bar 75 μm. Cx40 immunoreactivity appears punctated in glomeruli (A, B) and renin-expressing cells (A) but line-shaped in the endothelium (B).

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: The Connexin40 A96S Mutation Causes Renin-Dependent Hypertension

    doi: 10.1681/ASN.2010101047

    Figure Lengend Snippet: Cx40A96S protein is expressed in the plasma membrane of renin-expressing cells. Immunoreactivity of Cx40 (red), renin (green), and α-smooth muscle actin (blue) in sections of homozygous Cx40A96S kidneys. G indicates glomerulus; bar 75 μm. Cx40 immunoreactivity appears punctated in glomeruli (A, B) and renin-expressing cells (A) but line-shaped in the endothelium (B).

    Article Snippet: Primary rabbit Cx40 antibodies (BioTrend, Cologne, Germany) were diluted 1:400 in blocking solution and the cells were incubated for 1 hour at room temperature.

    Techniques: Clinical Proteomics, Membrane, Expressing

    Wildtype Cx40 protein is expressed in the plasma membrane of renin-expressing cells. Immunoreactivity of Cx40 (red), renin (green), and α-smooth muscle actin (blue) in sections of a wild-type kidney. G indicates glomerulus; bar 75 μm. Cx40 immunoreactivity appears punctated in intra- and extraglomerular mesangial cells and renin-expressing cells but line-shaped in the endothelium.

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: The Connexin40 A96S Mutation Causes Renin-Dependent Hypertension

    doi: 10.1681/ASN.2010101047

    Figure Lengend Snippet: Wildtype Cx40 protein is expressed in the plasma membrane of renin-expressing cells. Immunoreactivity of Cx40 (red), renin (green), and α-smooth muscle actin (blue) in sections of a wild-type kidney. G indicates glomerulus; bar 75 μm. Cx40 immunoreactivity appears punctated in intra- and extraglomerular mesangial cells and renin-expressing cells but line-shaped in the endothelium.

    Article Snippet: Primary rabbit Cx40 antibodies (BioTrend, Cologne, Germany) were diluted 1:400 in blocking solution and the cells were incubated for 1 hour at room temperature.

    Techniques: Clinical Proteomics, Membrane, Expressing